Novel system for isolating and identifying eukaryotic cells...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/79 (2006.01) C07K 16/00 (2006.01) C12N 15/13 (2006.01) C12N 15/18 (2006.01) C12N 15/62 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12Q 1/24 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2202907

The present invention relates to a novel expression system which allows the study of experimental genes of interest on cellular events soon after transfection. The expression system includes a vector which encodes for a recombinant antibody binding unit (rAb). The expression system enables identification and selection of transfected cells from culture to be carried out immediately, within hours, after the transfection event. The invention also relates to cells transfected with the expression system and methods for selection and isolation of cells transfected with the expression system.

La présente invention concerne un nouveau système d'expression qui permet d'étudier des gènes expérimentaux intéressants sur des évènements cellulaires peu après la transfection. Ce système d'expression comprend un vecteur qui code pour une unité de liaison à un anticorps de recombinaison (rAb). Ce système d'expression permet d'identifier et de sélectionner des cellules transfectées à partir de cultures immédiatement après la transfection, ou dans les quelques heures suivant la transfection. L'invention concerne également des cellules transfectées avec le système d'expression et les procédés pour sélectionner et isoler des cellules transfectées avec le système d'expression.

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