Novel t7 rna polymerase variants with enhanced thermostability

Details Novel t7 rna polymerase variants with enhanced thermostability Novel t7 rna polymerase variants with enhanced thermostability

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12N 15/00 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2737014

The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.

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