C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/54 (2006.01) C12N 1/20 (2006.01) C12N 9/56 (2006.01)
Patent
CA 2348295
Bacillus sp. NTAP-1 having been deposited under accession number FERM BP-6926; and a collagen-decomposing enzyme produced by bacterium. The above enzyme (1) has a capability of hydrolyzing, at the highest efficiency, collagen and gelatin from among casein, gelatin, albumin and collagen; (2) shows the optimum pH of 3.5 to 4.5; (3) shows the optimum temperature of 65 to 70°C; (4) after heating at 60°C at pH 6.0 for 4 hours, sustains an activity amounting to 60% or more of the level before the heat treatment; (5) remains stable at pH 3 to 6; and a molecular weight of approximately 46,000 when measured by SDS-PAGE.
L'invention concerne <i>Bacillus sp.</i> NTAP-1, objet de la demande numéro FERM BP-6826, et une enzyme digérant le collagène produit par cette bactérie. L'enzyme de l'invention 1) peut hydrolyser, pour un rendement maximal, le collagène et la gélatine parmi la caséine, la gélatine, l'albumine et le collagène; 2) présente le pH optimal de 3,5 à 4,5; 3) présente la température optimale de 65 à 70 ·C; 4) présente, après chauffage à 60 ·C pendant 4 heures à un pH de 6,0, une activité correspondant à au moins 60 % de son niveau d'avant traitement thermique; 5) reste stable à un pH de 3 à 6; et 6) présente un poids moléculaire d'environ 46.000 mesurée par SDS-PAGE.
Akai Minoru
Nakayama Toru
Nishino Tokuzo
Tsuruoka Naoki
Japan Science And Technology Corporation
Smart & Biggar
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