Novel upases and uses thereof

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/37 (2006.01)

Patent

CA 2643408

The present invention to newly identified polynucleotide sequences comprising genes that encode novel lipolytic enzymes LIP01-LIP03. The LIP01 enzyme may be isolated from Magnaporthe grisae, the LIP02 and LIP03 may be obtained by mutating the polynucleotide sequence encoding for LIP01. The invention features the full length coding sequence of the novel gene, which is suitable for expression in a suitable host cell as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods of using these proteins in industrial processes, for example in baking industry, vegetable oil degumming, production or modification of food emulsifiers and the production of glucose from wheat gluten.

La présente invention concerne des séquences polynucléotidiques nouvellement identifiées qui comprennent des gènes codant pour de nouvelles enzymes lipolytiques LIP01 à LIP03. L'enzyme LIP01 peut être isolée à partir de Magnaporthe grisae, les LIP02 et LIP03 peuvent être obtenues par mutation de la séquence polynucléotidique codant pour la LIP01. L'invention présente la séquence codante entière du nouveau gène, qui est exprimable dans une cellule hôte adéquate, ainsi que la séquence d'acides aminés de la protéine fonctionnelle entière et d'équivalents fonctionnels du gène ou de la séquence d'acides aminés. L'invention concerne en outre des procédés d'utilisation de ces protéines dans des processus industriels, par exemple dans l'industrie de la boulangerie, le dégommage des huiles végétales, la production ou la modification d'émulsifiants alimentaires et la production de glucose à partir de gluten de blé.

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