Nuclear binding assay for steroid receptor functionality in...

Details Nuclear binding assay for steroid receptor functionality in... Nuclear binding assay for steroid receptor functionality in...

G - Physics

01

N

167/45, 150/8.5

G01N 33/566 (2006.01) G01N 33/567 (2006.01) G01N 33/574 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1256359

Abstract of the Invention A method for rapidly determining the presence of functional cellular steroid receptors by assaying a tissue sample for nuclear steroid binding is disclosed which comprises treating the tissue with collagenase, incubating the isolated cells with a labelled steroid capable of complexing said receptors and measuring the bound radioac- tivity and the DNA of the isolated cellular nuclei.

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