C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/90 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/65 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2225652
The present invention provides novel DNA constructs and methods for stably transforming the plastids of higher plants. The constructs described herein contain unique promoters that are transcribed by both nuclear encoded plastid polymerases and plastid encoded plastid polymerases. Use of the novel constructs of the invention facilitates transformation of a wider range of plant species and enables tissue specific expression of a transforming DNA in plastids of multicellular plants.
La présente invention concerne des produits de recombinaison d'ADN et des procédés nouveaux pour transformer de manière stable les plastes de plantes supérieures. Ces produits de recombinaison comprennent des promoteurs uniques qui sont transcrits tant par des polymérases plastidiales codées par des nucléotides que par des polymérases plastidiales codées par des plastes. L'utilisation des nouveaux produits de recombinaison de cette invention facilite la transformation d'une gamme plus étendue d'espèces végétales et permet l'expression de la tissulo-spécifique d'un ADN transformant dans les plastes des plantes multicellulaires.
Allison Lori A.
Hajdukiewicz Peter T.
Maliga Pal
Borden Ladner Gervais Llp
Rutgers University
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