Nuclear matrix protein fluid assay

Details Nuclear matrix protein fluid assay Nuclear matrix protein fluid assay

C - Chemistry, Metallurgy

07

K

C07K 14/00 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/18 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2122473

Disclosed is a method for detecting and quantitating soluble nuclear matrix proteins in body fluids and extracellular media. The method is useful for monitoring the viability of cells and tissue, for evaluating the progress of a disease or its treatment, and for evaluating the cytotoxicity of unknown compounds. Also disclosed are methods for inducing the release of nuclear matrix proteins insoluble form from cells.

On décrit un procédé de détection et d'évaluation quantitative des protéines matricielles nucléaires présentes dans des fluides corporels et des milieux extracellulaires. Le procédé est destiné à être utilisé pour surveiller la viabilité de cellules et de tissus pour évaluer la progression d'une maladie ou du traitement de celle-ci et pour évaluer la cytotoxicité de composés inconnus. On décrit également des procédés destinés à induire la libération de protéines matricielles nucléaires sous forme solubles à partir des cellules.

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