Nuclear reprogramming method

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2660123

A process for effectively generating safe induced pluripotent stem cells from somatic cells, comprising the step of introducing the following three genes: Oct family gene, Klf family gene, and Sox family gene into somatic cells, or the step of introducing a combination of the following two genes: Oct family gene and Sox family gene or a combination of the following two genes: Oct family gene and Klf family gene, and at least one kind of genes selected from the following three genes: L-Myc, Sall1, and Sall4 into somatic cells.

L'invention porte sur un procédé efficace de génération d'une cellule souche pluripotente artificielle sans danger à partir d'une cellule somatique. Le procédé comporte l'étape d'introduction dans la cellule somatique des trois gènes suivants : un gène de la famille Oct, un gène de la famille Klf et un gène de la famille Sox, ou bien l'étape d'introduction dans la cellule somatique de la combinaison des deux gènes suivants : un gène de la famille Oct et un gène de la famille Sox ou de la combinaison des deux gènes suivants : un gène de la famille Oct et un gène de la famille Klf et d'au moins un gène choisi parmi les trois gènes suivants : un gène L-Myc, un gène Sall1 et un SAll4.

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