C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/00 (2006.01) C12N 5/073 (2010.01) C12N 15/877 (2010.01) A01K 67/027 (2006.01) C12N 15/87 (2006.01) A61K 35/54 (2006.01)
Patent
CA 2277192
An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
L'invention concerne un procédé perfectionné de transfert nucléaire, consistant à transplanter des noyaux de cellules donneuses différenciées, dans des ovocytes énucléés et de la même espèce que les cellules donneuses. On utilise les unités de transfert nucléaire résultantes pour la multiplication de génotypes et de génotypes transgéniques, au moyen de la production de foetus et de descendants, et pour la production de cellules isogéniques à masse cellulaire intérieure cultivée (CICM), notamment des cellules souches ou embryonnaires isogéniques. Le procédé de l'invention facilite la production d'embryons, de foetus et de descendants mammifères transgéniques ou génétiquement manipulés, étant donné que la source de cellules différentiées des noyaux donneurs peut être modifiée génétiquement et reproductible de manière clonale.
Cibelli Jose
Golueke Paul
Jerry D. Joseph
Ponce de Leon F. Abel
Robl James
Gowling Lafleur Henderson Llp
University Of Massachusetts A. Public Institution Of Higher Education Of The Commonwealth Of
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