Nuclease-resistant rna aptamer inhibiting replication of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2670447

Disclosed a nuclease-resistant RNA aptamer for inhibiting the replication of HCV replicon. This aptamer is capable of binding specifically to HCV NS5B and inhibiting the proliferation of the HCV replicon, and is composed of at least one sequence selected from a group consisting of SEQ ID NOS. 1 to 4, in which a fluoro group is substituted for 2' -hydroxy of both U (uracil) and C (cytosine) bases, and SEQ ID NO. 4, which is tagged with a cholesteryl group at a 5' end and with idT at a 3' end. The RNA aptamer is useful in the diagnosis and treatment of HCV infection.

L'invention concerne un aptamère d'ARN résistant aux nucléases destiné à inhiber la réplication du réplicon du VHC. Ledit aptamère peut se lier spécifiquement à la polymérase NS5B du VHC et inhiber la prolifération du réplicon du VHC. Ledit aptamère est constitué d'au moins une séquence sélectionnée dans un groupe constitué des SEQ ID NO. 1 à 4, dans lesquelles un groupe fluoro est substitué à 2'-hydroxy de bases U (uracile) et C (cytosine), et d'une SEQ ID NO. 4, qui est marquée à l'aide d'un groupe cholestéryle au niveau d'une extrémité 5' et présente de l'idT au niveau de l'extrémité 3'. L'aptamère d'ARN est utile pour le diagnostic et le traitement d'une infection par le VHC.

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