Nucleation of ice

C - Chemistry – Metallurgy – 07 – J

Patent

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Details

C07J 9/00 (2006.01) A01N 1/02 (2006.01) F25C 1/00 (2006.01) F25C 3/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2064708

2064708 9101992 PCTABS00003 The undercooling of an aqueous medium (and therefore damage to a biological sample in the medium) can be minimised by inducing ice nucleation in the medium using cholesterol having a crystal structure which may be obtained by crystallising from methanol and/or acetic acid. Such crystalline cholesterol can be added to the cryopreservation medium (which may also contain a cryoprotectant) or can be coated onto an inner surface of an ampoule, cryopreservation tube or straw. The cholesterol can also be used to induce ice nucleation in the atmosphere to create hail or snow or artificial snow in aerosols of the aqueous medium for use on ski slopes.

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