Nucleic acid amplification and detection of mycobacterium...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2389523

Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.

L'invention concerne des oligonucléotides utilisés pour amorcer l'amplification in vitro d'acides nucléiques de séquences d'ARNr 16S ou d'ADN codant pour les séquences d'ARNr 16S de nombreuses espèces du genre Mycobacterium. L'invention concerne également des kits comprenant ces oligonucléotides. L'invention concerne également des méthodes de détection d'espèces Mycobacterium à l'aide des oligonucléotides dans l'amplification in vitro d'acides nucléiques.

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