C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2096013
2096013 9208800 PCTABS00013 Novel methods are provided for nucleic acid amplification by continuous, substantially isothermal, self-sustained sequence replication ("3SR") utilizing RNA-dependent DNA polymerase activity, DNA-dependent DNA polymerase activity, RNAse H activity and DNA-dependent RNA polymerase activity. In one of the methods, before enzymatic activities can be provided by only two enzymes, a reverse transcriptase and a DNA-dependent RNA polymerase. The methods may employ two or three enzymes to provide the necessary enzymatic activities. Thus, in certain of the methods, an exogenous source of RNAse H, such as E. coli RNAse H, is employed in combination with a reverse transcriptase and a DNA-dependent RNA polymerase. In other of the methods of the present invention, reaction media are employed in which the inherent RNAse H activity of retroviral reverse transcriptases is effective to provide high levels of amplification so that only two enzymes, reverse transcriptase and DNA-dependent RNA polymerase, are required. Novel compositions for carrying out the methods of the present invention are also provided.
Fahy Eoin David
Gingeras Thomas Raymond
Guatelli John Christopher
Kwoh Deborah Yantis
Whitfield Kristina Marie
Macrae & Co.
Siska Diagnostics Inc.
LandOfFree
Nucleic acid amplification by two-enzyme, self-sustained... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleic acid amplification by two-enzyme, self-sustained..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid amplification by two-enzyme, self-sustained... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1750546