Nucleic acid amplification in the presence of modified...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2658520

The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.

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