Nucleic acid amplification methods

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2463040

The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

L'invention concerne des dosages et des nécessaires permettant de réaliser ces dosages aux fins d'une détection rapide et automatisée d'agents pathogènes infectieux et de gènes normaux ou anormaux. L'invention concerne en outre des méthodes d'amplification générale d'ADN génomiques et d'ARNm totaux, et l'utilisation desdites méthodes d'amplification pour analyser l'expression d'un ARNm différencié.

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