Nucleic acid amplification using nicking agents

Details Nucleic acid amplification using nicking agents Nucleic acid amplification using nicking agents

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2492220

The present invention provides methods and kits for amplifying target nucleic acids (including whole genomes) using nicking agents. In certain aspects, the amplification does not require the use of any external oligonucleotide primers that are capable of annealing to a portion of the target nucleic acid. This invention is useful in many areas such as genetic disease diagnoses, forensic analyses and paleoarcheological studies.

La présente invention concerne des méthodes et des kits d'amplification d'acides nucléiques cibles (y compris des génomes entiers) à l'aide d'agent de synchronisation. Dans certains aspects, l'amplification ne nécessite pas d'utilisation d'une quelconque amorce oligonucléotidique externe d'annelage à une partie de l'acide nucléique cible. L'invention s'utilise dans de nombreux domaines tels que les diagnostics de maladies génétiques, les analyses légistes et les études paléoarchéologiques.

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