Nucleic acid amplification using non-standard bases

Details Nucleic acid amplification using non-standard bases Nucleic acid amplification using non-standard bases

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2513626

The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture.

L'invention concerne des méthodes, kits et compositions pour des réactions d'amplification d'acides nucléiques haute fidélité d'acides nucléiques cibles, tels que des produits d'amplification comprenant des bases non standard. Les réactions d'amplification peuvent être linéaires ou exponentielles. Dans certains modes de réalisation, la fidélité de la réaction d'amplification est suffisamment élevée pour qu'une majorité de produits d'amplifications conserve les bases non standard sur des sites spécifiques désignés par l'utilisateur après un nombre spécifié de cycles d'amplification. Dans certains modes de réalisation, le rapport de nucléoside triphosphates non standard est supérieur à la quantité de nucléoside triphosphates standard initialement présents dans le mélange réactionnel.

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