Nucleic acid amplification utilizing intermediate duplexes

Details Nucleic acid amplification utilizing intermediate duplexes Nucleic acid amplification utilizing intermediate duplexes

C - Chemistry, Metallurgy

07

H

C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2449968

This invention provides for a novel amplification procedure for nucleic acid. The method uses a wild type or mutant RNA polymerase designed to transcribe both deoxyribonucleotides and ribonucleotides (see figures 1-7).

L'invention concerne une nouvelle méthode d'amplification d'acides nucléiques. Ladite méthode consiste à utiliser une ARN polymérase de type sauvage ou mutante destinée à transcrire les désoxyribonucléotides et les ribonucléotides (voir figures 1-7).

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