C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07D 401/04 (2006.01) C07D 403/04 (2006.01) C07D 413/04 (2006.01) C07D 417/04 (2006.01) C07D 487/04 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01) C12P 19/34 (2006.01) G01N 33/557 (2006.01)
Patent
CA 2562780
Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
L'invention concerne des procédés d'analyse d'acides nucléiques consistant à mélanger un acide nucléique cible avec un colorant se fixant sur l'ADN-db pour former un mélange, à ajouter éventuellement une sonde non marquée à ce mélange, et à créer une courbe de fusion de l'acide nucléique cible en mesurant la fluorescence produite par le colorant fixant sur l'ADN-db pendant que le mélange est chauffé. L'invention concerne également des colorants destinés à l'analyse des acides nucléiques, et des procédés permettant de préparer ces colorants.
Dujols Virginie E.
Holden Joseph A.
Willmore-Payne Carlynn
Wittwer Carl T.
Zhou Luming
Idaho Technology Inc.
Sim & Mcburney
University Of Utah Research Foundation
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