Nucleic acid assay procedure

Details Nucleic acid assay procedure Nucleic acid assay procedure

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2119701

NUCLEIC ACID ASSAY PROCEDURE Abstract A method of detecting the presence of a target polynucleotide in a sample is disclosed. The method comprises the steps of hybridizing the target polynucleotide with a detector probe, the detector probe comprising a first oligonucleotide having a detectable group such as alkaline phosphatase attached to the 3' terminus thereof; hybridizing the target polynucleotide with a capture probe, the capture probe comprising a second oligonucleotide having a capture group such as biotin attached to the 5' terminus thereof; and detecting the hybridization of the detector probe and the capture probe to the target polynucleotide. Probes, probe sets, and kits useful for carrying out the invention are also disclosed.

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