Nucleic acid binding polypeptide library

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 40/08 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 50/00 (2006.01) G01N 33/68 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2290720

The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomised, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomised, and which polypeptides may be assembled after selection to form a multifinger zinc finger polypeptide.

L'invention concerne une bibliothèque de polypeptides à doigts de zinc, dans laquelle chaque polypeptide renferme plus d'un doigt de zinc, ces doigts de zinc ayant été au moins partiellement répartis au hasard. L'invention concerne également un ensemble de bibliothèques de polypeptides à doigts de zinc, codant des polypeptides à doigts de zinc superposés, chaque polypeptide renfermant plus d'un doigt de zinc ayant été au moins partiellement réparti au hasard, et ces polypeptides pouvant être assemblés après avoir été choisis pour former un seul polypeptide à plusieurs doigts de zinc.

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