Nucleic acid binding proteins

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/47 (2006.01) C07K 1/04 (2006.01) C07K 14/00 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2290717

The invention provides a method for preparing a nucleic acid binding protein of the zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence.

L'invention concerne une méthode de préparation d'une protéine de liaison d'acide nucléique de la classe des doigts à zinc Cys2-His2 capable de se lier à un quadruplet d'acide nucléique dans une séquence d'acide nucléique cible, la liaison à la base 4 du quadruplet par un motif de liaison d'acide nucléique de doigt à zinc alpha -hélicoïdal dans la protéine étant déterminée comme suit: si la base 4 dans le quadruplet est A, alors la position +6 dans l'hélice alpha est Glu, Asn ou Val; si la base 4 dans le quadruplet C, alors la position +6 dans l'hélice alpha est Ser, Thr, Val, Ala, Glu ou Asn.

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