Nucleic acid construct for the cell cycle regulated...

Details Nucleic acid construct for the cell cycle regulated... Nucleic acid construct for the cell cycle regulated...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2224123

The invention refers to a nucleic acid construct comprising at least one activator sequence, at least one chimeric promoter module comprising a nucleotide sequence which binds a protein of the E2F family and a protein of the CDF-1 family, and at least one structural gene, wherein said chimeric promoter modul causes an up-regulation of gene expression in the cell cycle later than the B-myb promoter but earlier than the cdc25C promoter, and the protein CDF-1.

L'invention concerne un construit d'acide nucléique comprenant au moins une séquence activatrice, au moins un module promoteur chimère comprenant une séquence nucléotidique qui lie une protéine de la E2F et une protéine de la famille CDF-1, et au moins un gène structural, dans lequel ledit module promoteur chimère entraîne une régulation en amont de l'expression du gène au cours du cycle cellulaire plus tard que le promoteur B-myb, mais plus tôt que le promoteur cdc25C, et la protéine CDF-1.

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