Nucleic acid constructs

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) A61K 9/16 (2006.01) A61K 48/00 (2006.01) C12N 15/12 (2006.01) C12N 15/17 (2006.01) C12N 15/36 (2006.01) C12N 15/38 (2006.01)

Patent

CA 2542288

A nucleic acid construct comprising a chimeric promoter sequence and a cloning site for insertion of a coding sequence in operable linkage with the chimeric promoter, wherein the chimeric promoter sequence comprises: (a) a hCMV immediate early promoter sequence; (b) exon 1 and at least a part of exon 2 of the hCMV major immediate early gene; and (c) a heterologous'intron provided in place of the intron A region of the hCMV major immediate early gene.

L'invention concerne une construction d'acide nucléique comprenant une séquence promoteur chimère et un site de clonage destiné à l'insertion d'une séquence de codage liée de manière fonctionnelle au promoteur chimère, ladite séquence promoteur chimère comprenant : (a) une séquence promoteur précoce immédiate hCMV ; (b) l'exon 1 et au moins une partie d'exon 2 du gène précoce immédiat majeur hCMV ; et (c) un intron hétérologue présent à la place de la région d'intron A du gène précoce immédiat majeur hCMV.

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