Nucleic acid constructs

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/245 (2006.01) A61K 9/16 (2006.01) A61K 39/145 (2006.01) A61K 39/39 (2006.01) C12N 15/31 (2006.01) C12N 15/36 (2006.01) C12N 15/38 (2006.01) C12N 15/44 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2596731

A nucleic acid construct comprising a chimeric promoter sequence and a cloning site for insertion of a coding sequence in operable linkage with the chimeric promoter, wherein the chimeric promoter sequence comprises: (a) a Hcmv immediate early promoter sequence; (b) exon 1 and at least a part of exon 2 of the hCMV major immediate early gene; and (c) a heterologous intron provided in place of the intron A region of the hCMV major immediate early gene.

La présente invention concerne une construction d'acide nucléique comportant une séquence de promoteur chimère et un site de clonage pour l'insertion d'une séquence de codage en liaison fonctionnelle avec le promoteur chimère, ladite séquence du promoteur chimère comprenant: (a) une séquence promoteur précoce immédiate Hcmv; (b) l'exon 1 et au moins une partie de l'exon 2 du gène précoce immédiat majeur hCMV; et (c) un intron hétérologue présent à la place de la région d'intron A du gène précoce immédiat majeur hCMV.

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