C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) A61K 31/7088 (2006.01) A61K 48/00 (2006.01) C07K 1/14 (2006.01) C07K 14/62 (2006.01) C12N 15/17 (2006.01) C12N 15/63 (2006.01) C12P 21/00 (2006.01) A61K 35/12 (2006.01)
Patent
CA 2447658
Unique multifunctional nucleotide expression cassettes comprising a nucleotide sequence encoding a human protease driven by a glucose-regulatable promoter, a nucleotide sequence encoding human proinsulin having sites at its B-C and C-A junctions altered to permit cleavage by the human protease, and a CMV promoter driving the transcription of the nucleotide sequence encoding the human proinsulin, such cassettes optionally having polyadenylation sequences after each structural gene and the nucleotide sequence encoding human proinsulin optionally carrying an H10D variant, and cells transformed thereby.
L'invention concerne des cassettes d'expression de nucléotides multifonctionnelles uniques comportant une séquence de nucléotide codant une protéase humaine entraînée par un promoteur de régulation par le glucose, une séquence de nucléotide codant de la proinsuline humaine possédant des sites à ses jonctions B-C et C-A modifiées afin de permettre un clivage par la protéase humaine et un promoteur CMV entraînant la transcription de la séquence de nucléotide codant la proinsuline humaine, ces cassettes possèdent éventuellement des séquences de polyadénylation après chaque gène structurel et la séquence de nucléotide codant la proinsuline humaine portant éventuellement un variant H10D et des cellules ainsi transformées.
Barton Randall Wilber
Schneiderman Richard
Tatake Revati J.
Boehringer Ingelheim Pharmaceuticals Inc.
Fetherstonhaugh & Co.
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