Nucleic acid detection method by triple helix formation

Details Nucleic acid detection method by triple helix formation Nucleic acid detection method by triple helix formation

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2335276

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising (a) amplifying said target nucleic acid so that the product of the amplification reaction includes a purine rich region, (b) contacting the sample with a peptide nucleic acid able to bind to at least a portion of said target sequence; and (c) detecting the presence of triplex DNA structures. The detection is suitably effected directly, for example using a surface plasmon resonance detector.

L'invention concerne une méthode de détection de la présence d'une séquence d'acide nucléique cible dans un échantillon, la méthode consistant: (a) à amplifier l'acide nucléique cible de sorte que le produit de la réaction d'amplification comprenne une région riche en purine, (b) à mettre en contact l'échantillon avec un acide nucléique de peptide capable de se lier à au moins une partie de cette séquence cible; et (c) à détecter la présence de structures d'ADN triplex. La détection s'effectue directement de manière adéquate, par exemple au moyen d'un détecteur par résonance plasmonique de surface.

Affiliated with

Also associated with

Rating

Nucleic acid detection method by triple helix formation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid detection method by triple helix formation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid detection method by triple helix formation will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1952961