C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/12 (2006.01) C07H 21/00 (2006.01) C07K 14/47 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01) G01N 23/20 (2006.01)
Patent
CA 2313243
Disclosed here is a nucleic acid sequence encoding a recognition component of the N-end rule pathway. This nucleic acid sequence is characterized by the ability to specifically hybridize to the nucleic acid sequence of SEQ ID NO 1 under stringent hybridization conditions. Such conditions are defined below. Also disclosed is a nucleic acid sequence encoding a recognition component of the N-end rule pathway which is characterized by the ability to specifically hybridize to the nucleic acid sequence of SEQ ID NO 2 under stringent hybridization conditions. Also disclosed are DNA expression vectors containing nucleic acid sequences of the type described above, as well as cells transformed with such expression vectors. Further disclosed are applications for the compositions described above.
L'invention porte sur une séquence d'acide nucléique codant pour un composant reconnaissant la voie de la règle de l'extrémité N. Ladite séquence se caractérise par sa capacité à s'hybrider spécifiquement à la séquence de l'acide nucléique SEQ ID NO 1 dans des conditions rigoureuses d'hybridation définies dans la description. L'invention porte également: sur une séquence d'acide nucléique codant pour un composant reconnaissant la voie de la règle de l'extrémité N de d'acide nucléique SEQ ID NO 2 dans des conditions rigoureuses d'hybridation; sur des vecteurs d'expression d'ADN contenant les séquences d'acide nucléiques décrites ci-dessus; sur des cellules transformées à l'aide desdits vecteurs d'expression; et sur des applications des compositions décrites ci-dessus.
Kwon Yong Tae
Varshavsky Alexander
California Institute Of Technology
Goudreau Gage Dubuc
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