Nucleic acid encoding n-methylputrescine oxidase and uses...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) A01H 1/04 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/06 (2006.01) C12N 15/09 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2656430

The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.

L'invention concerne un gène codant la N-méthylputrescine oxydase (MPO) et des constructions comprenant un tel ADN, y compris des procédés de régulation de l'expression de la MPO, indépendamment ou avec d'autres gènes de la biosynthèse d'alcaloïde pour moduler la production d'alcaloïdes dans des plantes ou des cellules hôtes. Les gènes MPO ou leurs fragments sont utiles pour réduire la production d'alcaloïde pyrrolidine ou tropane dans des plantes, pour augmenter la production d'alcaloïde pyrrolidine ou tropane dans des plantes, et pour produire une enzyme MPO dans des cellules hôtes.

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