Nucleic acid enzyme light-up sensor utilizing invasive dna

G - Physics – 01 – N

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G01N 33/53 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2586006

The present invention provides a colorimetric light-up sensor for determining the presence and optionally the concentration of an analyte in a sample. Methods of utilizing the sensor and kits that include the sensor also are provided. The sensor utilizes invasive DNA to assist the analyte dependent disaggregation of an aggregate that includes nucleic acid enzymes, substrates, and particles.

La présente invention porte sur un capteur lumineux colorimétrique servant à déterminer la présence et éventuellement la concentration d'un analyte dans un échantillon. Cette invention concerne également des procédés d'utilisation de ce capteur et des trousses qui comprennent ce capteur. Le capteur de cette invention fait appel à un ADN invasif pour aider la désagrégation dépendante d'un analyte d'un agrégat qui comprend des enzymes d'acides nucléiques, des substrats et des particules.

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