Nucleic acid extraction on curved glass surfaces

Details Nucleic acid extraction on curved glass surfaces Nucleic acid extraction on curved glass surfaces

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12M 1/24 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2742598

Processes for extracting nucleic acid from a biological sample and related assemblies and kits are disclosed. The processes comprise the steps of (a) providing a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface is composed of unmodified, smooth glass and defines a tubular lumen providing fluid communication between the first port and second port, wherein the lumen is circular, oval, or elliptical in cross-section, and wherein the lumen is essentially free of nucleic acid-specific binding sites; (b) introducing a nucleic acid-containing sample into the lumen of the device via the first port; (c) allowing nucleic acid in the sample to bind to the unmodified smooth glass surface; and (d) washing the bound nucleic acid.

La présente invention concerne des procédés d'extraction de l'acide nucléique depuis un échantillon biologique et des ensembles et trousses associés. Les procédés comprennent les étapes suivantes : (a) la mise à disposition d'un dispositif comportant une surface interne, une surface externe, un premier orifice, et un second orifice, la surface interne étant constituée de verre lisse non modifié et définissant une lumière tubulaire assurant une communication fluidique entre le premier orifice et le second orifice, la lumière étant circulaire, ovale ou elliptique en section transversale, et la lumière étant sensiblement exempte de site de liaison spécifique à l'acide nucléique; (b) l'introduction d'un échantillon contenant de l'acide nucléique dans la lumière du dispositif via le premier orifice; (c) la liaison de l'acide nucléique avec la surface de verre lisse non modifiée; et (d) le lavage de l'acide nucléique lié.

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