Nucleic acid field effect transistor

G - Physics – 01 – N

Patent

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Details

G01N 27/414 (2006.01) C12Q 1/68 (2006.01) G01N 27/327 (2006.01)

Patent

CA 2456765

A method for electronically detecting hybridization of a probe nucleic acid and a target nucleic acid is disclosed. The probe nucleic acid (130) is attached to an open semiconductor channel (110) in a back-gated field effect transistor (120). A target nucleic acid is provided on the semiconductor channel, and electrical charateristics, such as the drain to source current, are monitored for changes indicating that hybridization has occured.

L'invention concerne un procédé permettant de détecter par voie électronique l'hybridation d'un acide nucléique sonde et d'un acide nucléique cible. Ledit acide nucléique sonde (130) est fixé à un canal semi-conducteur (110) ouvert dans un transistor à effet de champ à grille arrière (120). Un acide nucléique cible est amené dans le canal semi-conducteur, et des changements de caractéristiques électriques, telles que le courant drain-source, indiquant que l'hybridation a eu lieu sont surveillés.

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