C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
167/44, 150/8.5
C12Q 1/68 (2006.01) C07K 16/18 (2006.01) C12Q 1/70 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1238575
ABSTRACT OF THE DISCLOSURE Nucleic acid hybridization assay methods and reagent systems for detecting a particular polynucleo- tide sequence in a test medium. An aggregate is formed in the assay reaction mixture comprising intercalation complexes between a nucleic acid intercalator and double stranded nucleic acid associated with the hy- bridization product of the sequence to be detected and a nucleic acid probe sequence. Hybridization of the probe with the sequence to be detected can then be determined by addition of an antibody, or a fragment thereof, capable of binding with the intercalation complexes in the formed aggregate and measuring the antibody or fragment thereof which becomes bound to such intercalation complexes associated with hybri- dized probe. In one preferred embodiment, this method eliminates the need to chemically modify the probe in order to form a labeled reagent. In another embodi- ment, the method provides an advantageous method for labeling the probe by chemical modification. MS-1320-CIp-II
469908
Albarella James P.
Anderson Leslie H.d.
Miles Laboratories Inc.
Osler Hoskin & Harcourt Llp
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