Nucleic acid isolation

Details Nucleic acid isolation Nucleic acid isolation

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 1/08 (2006.01) C07H 21/00 (2006.01) B01D 15/38 (2006.01)

Patent

CA 2410888

The present invention relates to a method of isolating nucleic acid from a blood sample, said method comprising: (a) selectively isolating leucocytes from said sample by binding said leucocytes to a solid support by means of a binding partner specific for leucocytes; (b) lysing said isolated leucocytes; and (c) binding nucleic acid released from said lysed cells to said solid support. Kits for isolating nucleic acid from samples form further embodiments of the invention.

L'invention concerne un procédé d'isolation d'un acide nucléique provenant d'un échantillon de sang. Ledit procédé consiste à : (a) isoler de manière sélective des leucocytes dudit échantillon en liant lesdits leucocytes à un support solide au moyen d'un partenaire spécifique pour les leucocytes; (b) lyser lesdits leucocytes isolés; et (c) lier l'acide nucléique diffusé par lesdites cellules lysées audit support solide. Des assemblages permettant d'isoler l'acide nucléique des échantillons constituent les autres modes de réalisation de la présente invention.

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