Nucleic acid isolation methods and materials and devices...

Details Nucleic acid isolation methods and materials and devices... Nucleic acid isolation methods and materials and devices...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2597919

The present invention relates to methods for purifying nucleic acid from a sample using mild conditions that do not affect the chemical integrity of the nucleic acid. The method comprises contacting the sample with an matrix entrapped chitosan solid phase which is able to bind the nucleic acids at a first pH, and then extracting the nucleic acid from the solid phase by using an elution solvent at a second pH.

L'invention concerne des procédés permettant de purifier un acide nucléique provenant d'un échantillon dans des conditions douces qui n'affectent pas l'intégrité chimique de l'acide nucléique. Le procédé de l'invention consiste à mettre en contact l'échantillon avec une phase solide de chitosane piégée dans une matrice pouvant se lier aux acides nucléiques à un premier pH, puis à extraire l'acide nucléique de la phase solide au moyen d'un solvant d'élution à un deuxième pH.

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