Nucleic acid isolation using polidocanol and derivatives

Details Nucleic acid isolation using polidocanol and derivatives Nucleic acid isolation using polidocanol and derivatives

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12N 1/06 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2614069

This invention relates to a composition comprising a chaotropic agent, a buffering substance, and 0.5 to 5%(V/V) polidocanol or a derivative thereof. The invention is further related to uses of this composition and to a kit comprising the composition according to the invention. The invention is further related to a method for the detection of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5 % (V/V) polidocanol or a derivative thereof, optionally isolating the nucleic acid, optionally amplifying the nucleic acid, and detecting the nucleic acid. The invention is further related to a method for the purification of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5%(V/V) polidocanol or a derivative thereof and isolating the nucleic acid thereby purifying the nucleic acid.

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