Nucleic acid labeling by thermoanaerobacter...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/12 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2469928

An enzymatically active DNA polymerase or active fragment thereof, having at least 80 % identity in its amino acid sequence to the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus or fragment thereof, and having an amino acid alteration at position 720 in Tts Pol I or at position 426 in .DELTA.Tts Pol I or at a homologous position defined with respect to Tts DNA polymerase I, having improved incorporation of nucleotide analogs and natural bases during DNA synthesis compared to unaltered enzyme.

L'invention concerne une ADN polymérase active sur le plan enzymatique ou un fragment actif de celle-ci, possédant une séquence d'acide aminé identique à au moins 80 % à celle de l'ADN polymérase de Thermoanaerobacter thermohydrosulfuricus ou à un fragment de celle-ci, et possédant une modification d'acide aminé au niveau de la position 720 dans Pol I de Tts ou au niveau de la position 426 dans .DELTA. Pol I de Tts ou à une position homologue définie par rapport à l'ADN polymérase I de Tts, présentant une incorporation améliorée d'analogues de nucléotide et de bases naturelles pendant la synthèse de l'ADN, comparativement à une enzyme non modifiée.

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