Nucleic acid ligands and improved methods for producing the...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 19/06 (2006.01) C07H 19/10 (2006.01) C07H 21/00 (2006.01) C07K 14/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12Q 1/37 (2006.01) G01N 33/532 (2006.01) G01N 33/535 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01) C07B 61/00 (2006.01)

Patent

CA 2169536

The present invention describes methods for the identification and production of nucleic acid ligands to a target molecule based on the SELEX process. The methods may be performed with modified nucleic acids, blended nucleic acids and with separate steps of contacting of the candidate mixture of nucleic acids with target and with nontarget molecules. Included in the invention are methods for the identification and preparation of nucleic acid ligands to thrombin, vascular endothelial growth factor, human neutrophil elastase, caffeine and theophylline and nucleic acid ligands to thrombin identified by the SELEX method.

L'invention concerne des procédés d'identification et de production d'acides nucléiques servant de ligands avec une molécule cible par le procédé SELEX (évolution systématique de ligands par enrichissement exponentiel). On peut utiliser de tels procédés avec des acides nucléiques modifiés ou mélangés, selon des phases séparées destinées à mettre le mélange d'acides nucléiques condidat en contact avec des molécules cibles et non cibles. L'invention englobe des procédés d'identification et de préparation d'acides nucléiques servant de ligands avec la thrombine, le facteur de croissance endothéliale vasculaire, l'élastase de granulocytes neutrophiles humains, la caféine et la théophylline, ainsi qu'avec la thrombine quand ils sont identifiés par le procédé SELEX.

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