Nucleic acid ligands that bind to and inhibit dna polymerases

Details Nucleic acid ligands that bind to and inhibit dna polymerases Nucleic acid ligands that bind to and inhibit dna polymerases

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12N 9/99 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2223078

This invention discloses high-affinity oligonucleotide ligands to the thermostable Taq polymerase and Tth polymerase. Specifically, this invention discloses DNA ligands having the ability to bind to the Taq and Tth polymerases and the methods for obtaining such ligands. The ligands are capable of inhibiting polymerases at ambient temperatures.

La présente invention concerne des ligands nucléotidiques de haute affinité avec la Taq polymérase et la Tth polymérase thermostables. Spécifiquement, elle se rapporte à des ligands d'ADN capables de se fixer à la Taq polymérase et la Tth polymérase ainsi que les procédés permettant d'obtenir ces derniers. Lesdits ligands sont capables d'inhiber des polymérases à température ambiante.

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