Nucleic acid linkers and their use in gene synthesis

Details Nucleic acid linkers and their use in gene synthesis Nucleic acid linkers and their use in gene synthesis

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2468235

The invention relates to a single-stranded nucleic acid molecule used in a method for the production of a nucleic acid. The nucleic acid molecule comprises part A and part B. Part A comprises a sequence which corresponds at least to one partial sequence of the recognition sequence of a restriction enzyme of type IIS and part B comprises any particular but defined sequence of nucleotides. By using said nucleic molecules it is possible to link various partial sequences in a sequence-independent manner and thus carry out effective synthesis of a nucleic acid by resorting to standardized elements.

La présente invention concerne une molécule d'acide nucléique simple brin destinée à être utilisée dans un procédé de production d'un acide nucléique. Cette molécule d'acide nucléique comprend une partie A et une partie B, la partie A comprenant une séquence correspondant à au moins une séquence partielle de la séquence de reconnaissance d'une enzyme de restriction de type IIS et la partie B comprenant une suite de nucléotides quelconque, mais définie. L'utilisation de ce type de molécule d'acide nucléique permet de lier différentes séquences partielles indépendamment des séquences et ainsi de procéder de manière efficace à la synthèse d'un acide nucléique en ayant recours à des éléments standardisés.

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