C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) B09C 1/00 (2006.01) B09C 1/10 (2006.01) C12N 1/26 (2006.01) C12N 15/10 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/554 (2006.01) G01N 33/60 (2006.01)
Patent
CA 2352500
Novel and useful nucleic acids that can be used for detection of chlorinated ethylene-decomposing bacteria and preferentially hybridize to the 16S rRNA or rDNA of chlorinated ethylene-decomposing bacteria, and a method of detection of chlorinated ethylene-decomposing bacteria and a method of decomposition of chlorinated ethylene or ethane using these nucleic acids are disclosed, wherein PCR is performed using nucleic acid comprising 18 ~ 25 nucleotides that preferentially hybridizes to the 16S rRNA or rDNA of chlorinated ethylene-decomposing bacteria and has any of base sequences No. 1 ~ 1 5, a base sequence that has at least 90% homology with any of these base sequences, or a base sequence complementary to any of these base sequences as the primer and the nucleic acid in a sample as the template and the DNA fragment that has been synthesized is detected, and chlorinated ethylene or ethane can be decomposed by introducing the chlorinated ethylene-decomposing bacteria detected by this method to contaminated soil or underground water.
Nakamura Kanji
Ueno Toshihiro
Borden Ladner Gervais Llp
Kurita Water Industries Ltd.
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