Nucleic acid preparation

Details Nucleic acid preparation Nucleic acid preparation

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2527167

The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method allows to speed up overall reaction time without affecting the limit of detection.

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