Nucleic acid primers of acid-fast bacterium and method of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2403118

A technique, whereby a toxic acid-fast bacterium contaminating specimens such as drinks can be quickly and conveniently identified, comprising detecting the acid-fast bacterium in a specimen by the PCR method by using a pair of primers of a specific combination selected from among the nucleic acid primers having the base sequences represented by SEQ ID NOS:1 to 14, and then identifying the same.

L'invention concerne une technique permettant d'identifier de manière rapide et pratique une bactérie toxique résistant aux acides, contaminant des spécimens tels que des boissons. Cette technique consiste à détecter la bactérie résistant aux acides dans un spécimen par PCR, au moyen d'une paire d'amorces constituées d'une combinaison spécifique, sélectionnée parmi les amorces d'acides nucléiques présentant les séquences de base représentées par SEQ ID NO : 1 à 14, puis à l'identifier.

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