Nucleic acid probe sandwich assay methods and compositions

Details Nucleic acid probe sandwich assay methods and compositions Nucleic acid probe sandwich assay methods and compositions

C - Chemistry, Metallurgy

12

Q

195/1.12, 150/8.

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 1308372

ABSTRACT NUCLEIC ACID PROBE ASSAY METHODS AND COMPOSITIONS The invention provides nucleic acid probe assay methods, particularly sandwich assay methods, and compositions for use therein. The methods of the invention employ novel, oligonucleotide probe-derivatized solid supports, in which oligonucleotide probe, of 15-100 bases in a sequence complementary to a sequence of nucleic acid analyte, is terminally covalently bound. The solid supports of the invention are based on beads of amino-, sulfhydryl, or carboxyl-derivatized controlled pore glass, cyanogen bromide-treated cross-linked dextran, or polystyrene. The linkage between the terminally bound oligonucleotide and the support is effected through these groups, with which the support material is derivatized. The methods of the invention are based on several unexpected discoveries made in the course of investigating the properties of the novel solid supports of the invention: (A) There is little difference between carrying out a sandwich assay by (i) simultaneously hybridizing analyte to solid-support-bound probe and labeled probe in solution and (ii) first hybridizing analyte and labeled probe and then hybridizing analyte to solid support-bound probe. (B) The efficiency of capture of analyte is increased by prehybridizing probe- derivatized solid-support with non-homologous nucleic acid or dextran sulfate or both. (C) The presence of dextran sulfate in a hybridizing solution significant- ly increases the efficiency of capture of analyte by probe-derivatized solid support. The invention also entails test kits for nucleic acid probe sandwich assays with the solid supports of the invention.

544236

Affiliated with

Also associated with

Rating

Nucleic acid probe sandwich assay methods and compositions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid probe sandwich assay methods and compositions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid probe sandwich assay methods and compositions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1278703