Nucleic acid processing methods, kits and devices

Details Nucleic acid processing methods, kits and devices Nucleic acid processing methods, kits and devices

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12M 1/40 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2526409

Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.

Des échantillons biologiques contenant des acides nucléiques ARN et ADN, sont débarrassés de protéines liées par incubation avec un agent chaotrope tel qu'un sel de guanidium et le mélange est préparé pour un traitement ultérieur par dilution de cet agent à une concentration inférieure à 0,05 M sans que l'ARN et l'ADN soient physiquement isolés l'un de l'autre ou des autres composants du mélange de réaction. Des procédés incluent une telle préparation et un traitement supplémentaire, du type amplification et détection, qui peut être effectué dans un unique contenant. L'agent chaotrope peut être fourni en tant que réactif sec adhérant à un contenant. Les trousses de la présente invention peuvent contenir de tels réactifs, seuls ou associés à des réactifs d'amplification.

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