Nucleic-acid programmable protein arrays

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2563168

Arrays of polypeptides can be generated by translation of nucleic acid sequences encoding the polypeptides at individual addresses on the array. This allows for the rapid and versatile development of a polypeptide microarray platform for analyzing and manipulating biological information. In one embodiment, one or more nucleic acids that include a coding region and an anchoring agent are to stably attached to the substrate. The substrate can also be modified to include a binding agent.

Des réseaux de polypeptides peuvent être générés par translation de séquences d'acides nucléiques codant les polypeptides à des adresses individuelles sur le réseau. Ceci permet le développement rapide et polyvalent d'une plate-forme d'un micro-réseau de polypeptides, pour l'analyse et la manipulation d'informations biologiques. Suivant une forme d'exécution, un ou plusieurs acides nucléiques qui comprennent une région de codage et un agent d'ancrage peuvent être reliés de manière stable au substrat. Le substrat peut également être modifié de manière à inclure un agent de liaison.

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