Nucleic acid purification method

Details Nucleic acid purification method Nucleic acid purification method

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2664211

Disclosed is a process for separating and/or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

La présente invention concerne un procédé pour séparer et/ou purifier un acide nucléique par l'élution de l'acide nucléique à partir de résines anioniques dans des conditions de forte concentration de sel et en présence dans l'éluant d'un additif comprenant la guanidine ou un dérivé de type guanidine. Le procédé permet d'obtenir une bonne récupération d'acides nucléiques à partir de résines anioniques sans altérer la stabilité de l'acide nucléique par comparaison aux procédures chromatographiques d'échange ionique classiques.

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