Nucleic acid sequence amplification

Details Nucleic acid sequence amplification Nucleic acid sequence amplification

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2141430

A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autolytically under condi-tions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target se-quence autolytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. One of the blocking or modifying agents is alkane-diol which is shown in the figure. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar sam-ples, cloning and generating probes.

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