Nucleic acid sequences encoding a plant cytoplasmic protein...

Details Nucleic acid sequences encoding a plant cytoplasmic protein... Nucleic acid sequences encoding a plant cytoplasmic protein...

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C11B 1/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2177598

By this invention, a plant .beta.-ketoacyl-CoA synthase condensing enzyme is provided free from intact cells of said plant and capable of catalyzing the production of very long chain fatty acid molecules. Also contemplated are constructs comprising the nucleic acid sequence and a heterologous DNA sequence not naturally associated with the condensing enzyme encoding sequences, and which provide for at least transcription of a plant condensing enzyme encoding sequence in a host cell. In this fashion very long chain fatty acid molecules may be produced in a plant cell. Included are methods of modifying the composition of very long chain fatty acid molecules in a plant cell.

L'invention se rapporte à une enzyme de condensation d'une plante, la .beta.-cétoacyl-CoA-synthase, qui est dépourvue de cellules intactes de ladite plante et qui est apte à catalyser la production de molécules d'acide gras à très longue chaîne. Sont également décrits des produits de recombinaison comprenant la séquence d'acide nucléique ainsi qu'une séquence d'ADN hétérologue qui n'est pas naturellement associée aux séquences codant l'enzyme de condensation, et qui permettent au moins de transcrire une séquence codant une enzyme de condensation de plante dans une cellule hôte. De molécules d'acide gras à très longue chaîne peuvent ainsi être produites dans une cellule de plante. L'invention se rapporte également à des procédés de modification de la composition de molécules d'acide gras à très longue chaîne dans une cellule de plante.

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