Nucleic acid sequences encoding beta-ketoacyl-acp synthase...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/54 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A23D 9/00 (2006.01) C11B 1/00 (2006.01) C12N 1/13 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2412400

This invention relates to sequences encoding .beta.-ketoacyl- ACP synthase (KAS) and methods of use thereof. Also provided are methods for decreasing saturated fatty acid levels as a component of total triglycerides found in plant oils. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5' to 3' direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding a KAS protein, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with less than about 3.5 weight percent total saturated fatty acids.

La présente invention concerne des séquences codantes pour la .beta.-cétoacyl- ACP synthase (KAS) et des techniques d'utilisation de celles-ci. Cette invention concerne aussi des techniques permettant de diminuer les niveaux d'acides gras saturés sous forme de composant des triglycérides totales trouvées dans les huiles végétales. Cette technique comprend généralement un plant de soja ayant intégré dans son génome un construit d'ADN comprenant, dans les sens de transcription 5' et 3', un promoteur fonctionnel dans une cellule de graine de plant de soja, une séquence ADN codante pour une protéine KAS et une région de terminaison de transcription fonctionnelle dans une cellule végétale. La présente invention concerne aussi une graine de soja possédant un total de moins de 3,5 % en masse d'acides gras saturé.

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